One of the many challenges in the field of proteomics is separation of complex protein mixtures for quantitative and differential localization analysis. To directly address this issue, BioChain is pleased to offer the CNMCs Compartmental Protein Extraction Kit. This kit is designed for rapid and efficient sequential isolation of cytoplasmic, nuclear, membrane, and cytoskeletal proteins, all from the same tissue or cell samples. The system utilizes our proprietary buffers that have been optimized to prepare the highest quality compartmental protein extractions. The kit also includes our proprietary protease inhibitor cocktail to prevent protein degradation. In conjunction, these components result in protein extracts that may be used for downstream applications such as SDS-PAGE, isoelectric focusing and 2-D gel electrophoresis, Western blotting, gel mobility shift analysis, and other protein assays.